smoothelin (Novus Biologicals)

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Novus Biologicals smoothelin

( A ) To visualize SMCs, transverse sections of whole cervices from days 12, 15, 17, and 19 gravid mice ( n ≥ 3) were immunofluoresently stained with specific markers for a contractile (MYH11 and <t>SMTN)</t> or synthetic phenotype (RBP1 and MYH10). Sections were also stained with CNN1 and α-SMA, which are markers of contractile smooth muscle and myofibroblasts. The grayscale bar (left) is a schematic representation of markers associated with a particular SMC phenotype. Green indicates positive immunostaining for each marker; blue indicates nuclei counterstained with 4′,6-diamidino-2-phenylindole (DAPI). ( B ) Control immunoglobulin G (IgG; left) was used as a control for the primary antibodies, and primary antibodies were omitted as controls for the secondary antibodies. Representative dual immunostaining of MYH10 (green) and MYH11 (red). Scale bars, 1000 μm; inset scale bars, 60 μm. Inset depicts high-power view of select regions (dashed box) of interest, which show longitudinal- and circumferentially oriented contractile SMCs, as well as synthetic SMCs in the broad subepithelial region and diffusely throughout the stroma.

Smoothelin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+smoothelin/Smoothelin+Antibody/pmc11654679-263-41-43
Average 92 stars, based on 5 article reviews

smoothelin - by Bioz Stars, 2026-10

92/100 stars

Images

1) Product Images from "Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix"

Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix

Journal: Science Advances

doi: 10.1126/sciadv.adr3530

Figure Legend Snippet: ( A ) To visualize SMCs, transverse sections of whole cervices from days 12, 15, 17, and 19 gravid mice ( n ≥ 3) were immunofluoresently stained with specific markers for a contractile (MYH11 and SMTN) or synthetic phenotype (RBP1 and MYH10). Sections were also stained with CNN1 and α-SMA, which are markers of contractile smooth muscle and myofibroblasts. The grayscale bar (left) is a schematic representation of markers associated with a particular SMC phenotype. Green indicates positive immunostaining for each marker; blue indicates nuclei counterstained with 4′,6-diamidino-2-phenylindole (DAPI). ( B ) Control immunoglobulin G (IgG; left) was used as a control for the primary antibodies, and primary antibodies were omitted as controls for the secondary antibodies. Representative dual immunostaining of MYH10 (green) and MYH11 (red). Scale bars, 1000 μm; inset scale bars, 60 μm. Inset depicts high-power view of select regions (dashed box) of interest, which show longitudinal- and circumferentially oriented contractile SMCs, as well as synthetic SMCs in the broad subepithelial region and diffusely throughout the stroma.

Techniques Used: Staining, Immunostaining, Marker, Control

Isolation:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP244533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathwayrelated proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 °C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2-phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Staining:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Immunofluorescence:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Real-time Polymerase Chain Reaction:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Expressing:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Western Blot:

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction.
Article Snippet: Paraffin-embedded detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol.detrusor muscle samples were de-paraffinized with xylene and rehydrated via a graded series of ethanol. ... The indirect immunostainings of the tissue sections were performed at 4 ◦C overnight using the primary antibodies for the contractile proteins anti-α-SMA (1:200, NBP2-33006, Novus biologicals, CO, USA), anti-calponin (1:200, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:200, NBP2-37931, Novus biologicals, Centennial, CO, USA), anti-MyH11 (1:5, sc-6956, Santa Cruz, Heidelberg, Germany) as well as for the myostatin pathway-related proteins anti-myostatin (1:100, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (NBP2-54771, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:100, NBP1-57997, Novus biologicals, Centennial, CO, USA).. The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich, Switzerland) and goat anti-rabbit FITC (1:500, FP-SA500 Alexa 488, Brunschwig, Basel, Switzerland) and counter-stained with DAPI (4′,6-diamidino-2phenylindole, 1:200, D9542, Sigma Aldrich, Buchs, Switzerland).The slides were incubated with the secondary antibodies sheep anti-mouse Cy3 (1:700, C2181, Sigma Aldrich,..

Article Title: Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction
Article Snippet: A total of 1–1.5 mg/mL protein was used for WES analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany).analysis using a 12–230 kDa cartridge kit (Protein Simple WES, Germany). ... The primary antibodies for the contractile proteins anti-α-SMA (1:100, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:100, C2687, Sigma Aldrich, Buchs, Switzerland), anti-smoothelin (1:50, NBP2-37971, Novus, Centennial, CO, USA), anti-MyH11 (1:50, NBP2-44533, Novus biologicals, Centennial, CO, USA) as well as for the myostatin pathway-related proteins anti-myostatin (1:50, ab201954, Abcam, Cambridge, UK), anti-Smad 2 (NBP2-67376, Novus biologicals, Centennial, CO, USA), anti-p-Smad 2 (1:50, NBP2-54771, Novus biologicals, Centennial, CO, USA), anti-Smad 3 (1:50, NB100-56479, Novus biologicals, Centennial, CO, USA), anti-Smad 7 (1:50, MAB2029, Novus biologicals, Centennial, CO, USA) and anti-follistatin (1:50, AF669, R&D Systems, Minneapolis, USA) were used.. Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an internal control.Anti-GAPDH (1:100, B300-221, Novus Biologicals, Centennial, CO, USA) served as an..

Review